9,292
edits
BISC110: Series 4 Lab 11 Enzymology: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
no edit summary
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) No edit summary |
||
| Line 125: | Line 125: | ||
17. Wipe the outside of each test tube with a Kimwipe™ or if a film still remains on the tube, transfer the contents to a new 13mm test tube. Using tube #1 to blank the spectrophotometer, read the absorbance of each reaction at 540nm and record all data in your lab notebook. Were the activity levels of the invertase produced by the yeast cells higher or lower than the activity of the purified invertase? | 17. Wipe the outside of each test tube with a Kimwipe™ or if a film still remains on the tube, transfer the contents to a new 13mm test tube. Using tube #1 to blank the spectrophotometer, read the absorbance of each reaction at 540nm and record all data in your lab notebook. Were the activity levels of the invertase produced by the yeast cells higher or lower than the activity of the purified invertase? | ||
=='''Laboratory Clean up== | |||
<br> | |||
Pour the DNS solutions in the waste jar in the hood. Discard the empty test tubes from the invertase assay in the glass recycle container at the front of the lab. You may want to wear gloves to avoid yellow stains on your hands. All materials contaminated with bacteria should be discarded in the orange autoclave bags taped at the benches. | |||
