BISC110: Series 4 Lab 11 Enzymology: Difference between revisions

BISC110: Series 4 Lab 11 Enzymology (view source)

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 .  Each reaction should proceed for 15min exactly.  When the time is almost up, take a 5mL pipette, your timer, your notebook, and the DNS reagent from your bench to the water bath. Stop each reaction by adding 3mL of DNS reagent to each tube at 30sec intervals, so that each tube will have reacted for 15min.  Please use caution so that you do not add DNS reagent to someone else's tubes.
-.  DNS reagent must react under alkaline conditions and the color change becomes evident only after the samples have been heated for 10min at
+.  DNS reagent must react under alkaline conditions and the color change becomes evident only after the samples have been heated for 10min at 90–95°C.  Place all your reaction tubes into one of the hot water baths that are contained in the chemical hoods in the lab.  Set your timer to count down for 10min, so you will know when the time is up.
-–95°C.  Place all your reaction tubes into one of the hot water baths that are contained in the chemical hoods in the lab.  Set your timer to count down for 10min, so you will know when the time is up.
 .  Carefully remove your tubes from the hot water bath.  There are test tube holders near the bath to help you with the hot tubes.  CAUTION: Take care not to burn your fingers in the hot water or with the steam, and please check that you are removing your own tubes from the bath.