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#Load appropriate protein molecular weight markers
#Fill the Lower (outer) Buffer Chamber with 600 ml of 1X NuPAGE® SDS Running Buffer
#Run the gel at 200 V constant. Run for 50 -55 minutes.(Check for bubbles emerging from solution to see if electrophoresis is running.)
'''Removing the Gel after Electrophorsis'''
#Put Kodak film over transfer membrane in cassette and close cassette. Leave for one minute. (Note: Do not expose film to light. Keep lights off until film is in imaging machine)
#Put film into imaging machine to process
#If nothing shows, put the film back into the cassette and leave it in for another 30 minutes. Check back to see if results show.


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