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Dorman:P22 transduction: Difference between revisions
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#To make the plates, at 62.5 mg Alizarin yellow (on Dan's bench) to the 100 mL bottle of green base, and place the agar in the steamer. | #To make the plates, at 62.5 mg Alizarin yellow (on Dan's bench) to the 100 mL bottle of green base, and place the agar in the steamer. | ||
#When the 100mL bottle comes out of the steamer and has been cooled in the 55° bath, add 0.33 mL of 2% | #When the 100mL bottle comes out of the steamer and has been cooled in the 55° bath, add 0.33 mL of 2% Aniline blue (on Dan's bench), 4.2 mL of 20% glucose, and appropriate antibiotics. | ||
