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Purification of DNA: Difference between revisions
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2-propanol (isopropanol) precipitation yields less DNA than EtOH precipitation. (In my hands 60% less on average! [[User:Jasu|Jasu]]) | 2-propanol (isopropanol) precipitation yields less DNA than EtOH precipitation. (In my hands 60% less on average! [[User:Jasu|Jasu]]) | ||
2-propanol at RT reduces the risk of co-precipitation of salts (which can interfere with downstream experiments). 2-propanol is preferred for large volumes of DNA since less alcohol is required, plus it's faster since it doesn't require a cooling step. Glassy pellets from 2-propanol precipitation are harder to see than fluffy, salt-containing pellets from ethanol precipitation. 2-propanol pellets are also more loosely attached after centrifugation. Take care when decanting. | 2-propanol at RT reduces the risk of co-precipitation of salts (which can interfere with downstream experiments). 2-propanol is preferred for large volumes of DNA since less alcohol is required, plus it's faster since it doesn't require a cooling step. Glassy pellets from 2-propanol precipitation are harder to see than fluffy, salt-containing pellets from ethanol precipitation. 2-propanol pellets are also more loosely attached after centrifugation. Take care when decanting. | ||
==Size-dependent precipitation by PEG8000/MgCl<sub>2</sub>== | |||
#[[Protocol Size selective DNA precipitation by PEG/MgCl2]] | |||
==Filtration / affinity columns== | ==Filtration / affinity columns== | ||
