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Dorman:P22 transduction: Difference between revisions
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'''Day 2''' | '''Day 2''' | ||
You should have colonies on some of your plates. To minimize the possibility of working with a double transductant, use colonies of the plate that used the most diluted P22. | You should have colonies on some of your plates. To minimize the possibility of working with a double transductant, use colonies of the plate that used the most diluted P22. | ||
While ''int'' P22 cannot form true lysogens, pseudolysogens can occur. We can distinguish phage-cured transducatants from pseudolysognens by streaking on green agar plates. Phage free colonies are light green, while psedolysogens are dark blue-green. | |||
#Streak a desired number of colonies onto green agar plates. I typically pick four for routine strain construction. | #Streak a desired number of colonies onto green agar plates. I typically pick four for routine strain construction. | ||
