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Ethanol precipitation of small DNA fragments: Difference between revisions
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Ethanol precipitation of small DNA fragments (view source)
Revision as of 01:20, 23 June 2008
, 23 June 2008→Flexibility Notes
Eric Meltzer (talk | contribs) |
Eric Meltzer (talk | contribs) |
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*In place of 95% EtOH for the wash, anything down to about 70% is acceptable. | *In place of 95% EtOH for the wash, anything down to about 70% is acceptable. | ||
*In place of a -80, a -40 has worked well for me | *In place of a -80, a -40 has worked well for me. | ||
*If a cooled centrifuge is unavailable, a normal one will usually also work (obviously with inferior results, but in my experience, even small fragments reclaimed this way have ligated well) | *If a cooled centrifuge is unavailable, a normal one will usually also work (obviously with inferior results, but in my experience, even small fragments reclaimed this way have ligated well) | ||
