Berglund:Materials: Difference between revisions
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120µg yeast pyrophosphatase from Sigma i1891 (0.01U/µl)= 120U lyophilized and would be diluted into a fv=12ml<br> | 120µg yeast pyrophosphatase from Sigma i1891 (0.01U/µl)= 120U lyophilized and would be diluted into a fv=12ml<br> | ||
But, you may receive less than 120µg and so you dilute accordingly (100µg =100U= fv of 10ml for 10x stock)<br> | But, you may receive less than 120µg and so you dilute accordingly (100µg =100U= fv of 10ml for 10x stock)<br> | ||
[[Category:Berglund_Protocols]][[Category:Berglund_Nucleic_Acid]][[Category:Berglund_Transcription]] | |||
Latest revision as of 18:07, 28 May 2008
10x yeast pyrophosphatase
50% glycerol
10mM HEPES-KOH pH 7.5
6mM MgCl2
120µg yeast pyrophosphatase from Sigma i1891 (0.01U/µl)= 120U lyophilized and would be diluted into a fv=12ml
But, you may receive less than 120µg and so you dilute accordingly (100µg =100U= fv of 10ml for 10x stock)
