3,675
edits
Phusion: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
no edit summary
Barry Canton (talk | contribs) |
Barry Canton (talk | contribs) No edit summary |
||
| Line 47: | Line 47: | ||
|} | |} | ||
A 2x supermix is now available containing either HF buffer or GC buffer, dNTPs, and Phusion polymerase. See references section below for links. | |||
==Thermocycling conditions== | ==Thermocycling conditions== | ||
#15-30 s/kb extension time. | #15-30 s/kb extension time. | ||
| Line 56: | Line 57: | ||
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase] | *[http://www.neb.com/nebecomm/products/productF-530.asp Purchase] | ||
*[http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf Manual] | *[http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf Manual] | ||
*[http://www.neb.com/nebecomm/products/productF-531.asp HF Master Mix product page] | |||
*[http://www.neb.com/nebecomm/products/productF-532.asp GC Master Mix product page] | |||
[[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:DNA]] | [[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:DNA]] | ||
