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Tetracycline: Difference between revisions
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→Working concentration and Stock Solutions
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*Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive. | *Stock solution is 15 mg/ml in 50% ethanol. Add 1 ml per liter of cooled broth or agar. Solutions and plates are light sensitive. | ||
*Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media | *Tetracycline is incompatible with (chelated by) magnesium ions, and thus unsuitable for use in minimal media | ||
*Fusaric acid can be used to select against tetracycline resistant colonies. See PMID 6259126, and modifications PMID 7007341. | |||
*Nickel ions can also be used for tetR counterselection. See PMID 8954882. | |||
==Plate Color Code== | ==Plate Color Code== | ||
