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'''Day 1''' | '''Day 1''' | ||
# Set up multiple transductions: | # Set up multiple transductions: | ||
* 100 μ of P22 lysate + 100μ of recipient strain | * 100 μL of P22 lysate + 100μL of recipient strain | ||
* 100 μ of 1:10 diluted P22 lysate + 100μ of recipient strain | * 100 μL of 1:10 diluted P22 lysate + 100μL of recipient strain | ||
* 100 μ of 1:100 diluted P22 lysate + 100μ of recipient strain | * 100 μL of 1:100 diluted P22 lysate + 100μL of recipient strain | ||
* 100μ of recipient strain (no phage control- confirms that there are no mutants in the recipient population) | * 100 μL of recipient strain (no phage control- confirms that there are no mutants in the recipient population) | ||
* 100 μ of P22 lysate (no recipent control- confirms that the lysate is not contaminated with bacteria.) | * 100 μL of P22 lysate (no recipent control- confirms that the lysate is not contaminated with bacteria.) | ||
#Incubate these mixtures at 37°C (no shaking necessary) for 1 hour. | #Incubate these mixtures at 37°C (no shaking necessary) for 1 hour. |
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