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Ampicillin: Difference between revisions
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==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
Expression of [[β-lactamase]] neutralizes ampicillin. The resistance gene is named ''bla'' or ''amp<sup>R</sup>''. When this enzyme is expressed on a high-copy number plasmid there is significant diffusion into the extracellular medium. As a result non-resistant satellite colonies may form around larger resistant colonies. | |||
Expression of [[β-lactamase]] neutralizes ampicillin. When this enzyme is expressed on a high-copy number plasmid there is significant diffusion into the extracellular medium. As a result non-resistant satellite colonies may form around larger resistant colonies. | |||
[[Image:Amp2.jpg|thumb|300px|left|Satellite colonies on an Ampicillin plate]] | [[Image:Amp2.jpg|thumb|300px|left|Satellite colonies on an Ampicillin plate]] | ||
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