In vitro transcription with T7 RNA polymerase: Difference between revisions

In vitro transcription with T7 RNA polymerase (view source)

3 bytes added , 17 June 2005

m

no edit summary

1,262

edits

@@ Line 34: / Line 34: @@
 '''Inorganic pyrophosphatase'''
-Inorganic pyrophosphatase cleave pyrophosphate (PO4-PO4), which is released when a nucleoside triphosphate is incorporated/polymerized, into phosphate (PO4). This helps to fight against any inhibitory effect of having pyrophosphate around (i.e. prevents the "reverse" reaction.) This is an optional component of the transcription reaction. If you leave it out, often you will see something precipitate (white) in your transcription reaction. This is the pyrophosphate.
+Inorganic pyrophosphatase cleaves pyrophosphate (PO4-PO4), which is released when a nucleoside triphosphate is incorporated/polymerized, into phosphate (PO4). This helps to prevent against any inhibitory effect of having pyrophosphate around (i.e. prevents the "reverse" reaction.) This is an optional component of the transcription reaction. If you leave it out, often you will see something precipitate (white) in your transcription reaction. This is the pyrophosphate.
 Make a 0.1 U/µL stock solution in H2O and store at -20 ˚C.