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(conc. of MnCl2-4H2O corrected) |
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==Materials== | ==Materials== | ||
* PIPES 0.5M solution pH 6.7 | * PIPES buffer 0.5M solution pH 6.7 (sterilized, made with milli-Q pure H2O, store at -20C) | ||
* Manganese chloride tetrahydrate | * Manganese chloride tetrahydrate | ||
* Calcium chloride dihydrate | * Calcium chloride dihydrate | ||
