132
edits
GHV-68 Plaque Assay: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Procedure
| Line 17: | Line 17: | ||
#Aliquot 2/ml/well (3-5x10<sup>5</sup> cells/well) into 6-well plates. Don't swirl the cells, as you may end up with all your 3T12's in the center of the well. | #Aliquot 2/ml/well (3-5x10<sup>5</sup> cells/well) into 6-well plates. Don't swirl the cells, as you may end up with all your 3T12's in the center of the well. | ||
##Need one 6-well plate per viral sample. (This will let you test 6 logs of dilution) | ##Need one 6-well plate per viral sample. (This will let you test 6 logs of dilution) | ||
#Prepare[[Methylcellulose]], if necessary. | #Prepare [[Methylcellulose]], if necessary. | ||
#Freeze/thaw/freeze your viral sample, if necessary. | #Freeze/thaw/freeze your viral sample, if necessary. | ||
