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GHV-68 Plaque Assay: Difference between revisions
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→Procedure
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==Procedure== | ==Procedure== | ||
Day 0 | Day 0 | ||
#Trypsinize 3T12's (1 confluent T175 will yield approx. 10-40x10<sup>6,</sup> cells) and resuspend at 2.5-2.5x10<sup>6< | #Trypsinize 3T12's (1 confluent T175 will yield approx. 10-40x10<sup>6,</sup> cells) and resuspend at 2.5-2.5x10<sup>6</sup>/ml in DMEM-10. | ||
#Aliquot 2/ml/well (3-5x10<sup>5</sup> cells/well) into 6-well plates. Don't swirl the cells, as you may end up with all your 3T12's in the center of the well. | #Aliquot 2/ml/well (3-5x10<sup>5</sup> cells/well) into 6-well plates. Don't swirl the cells, as you may end up with all your 3T12's in the center of the well. | ||
##Need one 6-well plate per viral sample. (This will let you test 6 logs of dilution) | ##Need one 6-well plate per viral sample. (This will let you test 6 logs of dilution) | ||
