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Ampicillin: Difference between revisions
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[[Image:Amp2.jpg|thumb|300px|left|Satellite colonies on an Ampicillin plate]] | [[Image:Amp2.jpg|thumb|300px|left|Satellite colonies on an Ampicillin plate]] | ||
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==Stock Solution== | |||
Typical concentrations of ampicillin are 50 ug/ml for low copy plasmids and 100 ug/ml for high copy plasmids. Stock solutions are typically at 100 mg/ml, so that 1 ml of antibiotic can be added to 1 liter of broth or agar. Stock solutions made in 50% alcohol remain liquid at -20 C and are easy to pipet. Cool agar to 55C or below prior to adding antibiotic. | |||
