Sauer:bis-Tris SDS-PAGE, the very best: Difference between revisions

Sauer:bis-Tris SDS-PAGE, the very best (view source)

2 bytes removed , 2 March 2022

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 Add water to 900 mLs.
-Carefully add glacial acetic acid to make the liter volume. The acid promoted Coomassie aggregation and sticking to other surfaces (like proteins!), so add it last to help the Coomassie dissolve well.
+Carefully add glacial acetic acid to make the liter volume. The acid promotes Coomassie aggregation and sticking to other surfaces (and proteins!), so add it last to help the Coomassie dissolve well.
 ====Destain====