257
edits
McKinney:7H9: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
no edit summary
(New page: ===Summary=== 7H9 (and its solid counterpart, 7H10 agar) are selective media used for the culture of free-living mycobacteria such as ''M. tuberculosis'' and ''M. smegma...) |
No edit summary |
||
| Line 4: | Line 4: | ||
===Ingredients=== | ===Ingredients=== | ||
(makes 1L medium) | |||
* Deionized water | * Deionized water | ||
| Line 16: | Line 18: | ||
For ''M. smegmatis'': | For ''M. smegmatis'': | ||
* 100mL ADS (Albumin-Dextrose-Saline): 8.1g NaCl, 50g Bovine Serum Albumin Fraction V, 20g anhydrous dextrose in 1L deionized water. Filter sterilize in a 0.22μm filter, and store at 4°C. | * 100mL ADS (Albumin-Dextrose-Saline): 8.1g NaCl, 50g Bovine Serum Albumin Fraction V, 20g anhydrous dextrose in 1L deionized water. Filter sterilize in a 0.22μm filter, and store at 4°C. | ||
===Protocol=== | |||
# Dissolve 4.7g Middlebrook 7H9 poweder in 900mL deionized water. Mix with a magnetic stir bar until soluble components have dissolved. | |||
# Add the rest of the ingredients appropriate for the organism at hand and continue stirring until well mixed. | |||
# Sterilize by filtration through a 0.22μm pore membrane. Store at 4°C. | |||
