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E. coli genotypes: Difference between revisions
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→Commonly used strains
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<biblio> | <biblio> | ||
#Garen-JMolBiol-1965 pmid=5327650 | #Garen-JMolBiol-1965 pmid=5327650 | ||
</biblio> | |||
===SG4044=== | |||
lon-100 Δ(gal-pgl)324 lac thi rpsL | |||
* StrR | |||
* Lon<sup>-</sup> | |||
* non-mucoid | |||
* The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology. | |||
* Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli. | |||
See [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20670 BCCM/GeneCorner Plasmid Collection #LMBP 670] | |||
<biblio> | |||
#Gottesman-JBacteriol-1981 pmid=7026534 | |||
#Remaut-NAR-1983 pmid=6308555 | |||
</biblio> | </biblio> | ||
