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E. coli genotypes: Difference between revisions
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→Commonly used strains
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** Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051. | ** Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051. | ||
** Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368. | ** Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368. | ||
===DH5αF=== | |||
F<sup>+</sup> ''endA1'' ''glnV44'' ''thi-1'' ''recA1'' ''relA1'' ''gyrA96'' ''deoR'' ''nupG'' ''purB20'' φ80d''lacZ''ΔM15 Δ(''lacZYA-argF'')U169, hsdR17(''r<sub>K</sub>''<sup>–</sup>''m<sub>K</sub>''<sup>+</sup>), λ<sup>–</sup> | |||
* This strain contains the F factor of NK3 and therefore can be used for infection with M13 or fd phages and derivatives. | |||
* Thiamine, arginine auxotroph | |||
* Lactose nonutilizing | |||
* Nalidixic acid resistant | |||
* Recombination-deficient | |||
* Relaxed phenotype | |||
* Endonuclease A deficient | |||
* Constitutive deoxyribose synthesis, good for cloning large plasmids. | |||
* See [http://bccm.belspo.be/catalogues/genecorner-hosts BCCM/GeneCorner Plasmid Collection #LMBP 1596] | |||
===DH5αF(λ)=== | |||
F<sup>+</sup> ''endA1'' ''glnV44'' ''thi-1'' ''recA1'' ''relA1'' ''gyrA96'' ''deoR'' ''nupG'' ''purB20'' φ80d''lacZ''ΔM15 Δ(''lacZYA-argF'')U169, hsdR17(''r<sub>K</sub>''<sup>–</sup>''m<sub>K</sub>''<sup>+</sup>), λ<sup>+</sup> | |||
* This strain contains the F factor of NK3 and therefore can be used for infection with M13 or fd phages and derivatives. | |||
* In addition the strain is lysogenic for wild-type phage λ; cultivate at 28°C to keep phage λ stably integrated. | |||
* Thiamine, arginine auxotroph | |||
* Lactose nonutilizing | |||
* Nalidixic acid resistant | |||
* Recombination-deficient | |||
* Relaxed phenotype | |||
* Endonuclease A deficient | |||
* Constitutive deoxyribose synthesis, good for cloning large plasmids. | |||
* See [http://bccm.belspo.be/catalogues/genecorner-hosts BCCM/GeneCorner Plasmid Collection #LMBP 2846] | |||
===DH5αpir=== | ===DH5αpir=== | ||
endA1 hsdR17 glnV44 (= supE44) thi-1 recA1 gyrA96 relA1 φ80dlacΔ(lacZ)M15 Δ(lacZYA-argF)U169 zdg-232::Tn10 uidA::pir+ | endA1 hsdR17 glnV44 (= supE44) thi-1 recA1 gyrA96 relA1 φ80dlacΔ(lacZ)M15 Δ(lacZYA-argF)U169 zdg-232::Tn10 uidA::pir+ | ||
* tetracyclin resistant | * tetracyclin resistant | ||
*See [http://bccm.belspo.be/catalogues/genecorner-hosts BCCM/GeneCorner Plasmid Collection #LMBP 7962] | * See [http://bccm.belspo.be/catalogues/genecorner-hosts BCCM/GeneCorner Plasmid Collection #LMBP 7962] | ||
<biblio> | <biblio> | ||
#Platt-Plasmid-1990 pmid=10610816 | #Platt-Plasmid-1990 pmid=10610816 | ||
