Sauer:bis-Tris SDS-PAGE, the very best: Difference between revisions

Sauer:bis-Tris SDS-PAGE, the very best (view source)

6 bytes added , 19 December 2019

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 This solution will contain meta-bisulfite. Depending on the vendor, your chemical powder may be labeled that way on the bottle to reflect its presence. It's is the right stuff.
-===3.5X gel buffer===
+===3X gel buffer===
-.25 M bis-Tris (pH 6.5-6.8 with HCl)
+.0 M bis-Tris (pH 6.5-6.8 with HCl)
 Note: <u>bis</u>-Tris is Bis(2-hydroxyethyl) aminotris (hydroxymethyl) methane (e.g. Sigma catalog# B 7535).
-We have changed our recipe slightly to make it easier to make and use.  Prepare 1.0 M bis-Tris as a "3X". Using thirds helps when making the gel solutions in graduated conical tubes (<i>e.g.</i>, pouring 15 mLs "by eye" to prepare 45 mLs of gel). Having the tank buffer and this gel buffer at pH 6.5 provides a more acidic environment and also more chloride ions than a pH 6.8 mixture, so try to get closer to 6.5, but don't sweat it.
+We have changed our recipe from the original to make it easier to make and use.  Prepare 1.0 M bis-Tris as a "3X". Using thirds helps when making the gel solutions in graduated conical tubes (<i>e.g.</i>, pouring 15 mLs "by eye" to prepare 45 mLs of gel). Having the tank buffer and this gel buffer at pH 6.5 provides a more acidic environment and also more chloride ions than a pH 6.8 mixture, so try to get closer to 6.5, but don't sweat it.
 ===sample buffer===