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QRT-PCR/Single tube: Difference between revisions
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→Starting Materials
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== Starting Materials == | == Starting Materials == | ||
* Validated PCR primers that are efficient over the range of RNA that you are assaying. | * Validated PCR primers & probe that are efficient over the range of RNA that you are assaying. | ||
* PCR primers and probe for a house keeping gene (ie B-actin, GAPDH, RPL19) | |||
* Taq Polymerase, MuLV RT | * Taq Polymerase, MuLV RT | ||
* dNTPs, MgCl2, nuclease free water | * dNTPs, MgCl2, nuclease free water | ||
