19
edits
QRT-PCR/Single tube: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Starting Materials
No edit summary |
|||
| Line 3: | Line 3: | ||
== Starting Materials == | == Starting Materials == | ||
* Validated PCR primers that are efficient over the range of RNA that you are assaying. | * Validated PCR primers that are efficient over the range of RNA that you are assaying. | ||
* | * Taq Polymerase, MuLV RT | ||
* dNTPs, MgCl2, nuclease free water | |||
* PCR strip caps or 96-well plates with transpearant caps | |||
* total RNA (~50ng per reaction, diluted to 10ng/ul) | |||
== Basic Principle == | == Basic Principle == | ||
