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Mssb18/ProtDesign: Difference between revisions
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* '''Study 6'''. Combinatorial library of a ranked ensemble of variants. | * '''Study 6'''. Combinatorial library of a ranked ensemble of variants. | ||
** 6.a Use the [[http://rosettadesign.med.unc.edu/ RosettaDesign]] webserver in order to perform a //saturation mutagenesis// focused library for the hot-spots positions. | ** 6.a Use the [[http://funclib.weizmann.ac.il/ FuncLib]] or the [[http://rosettadesign.med.unc.edu/ RosettaDesign]] webserver in order to perform a //saturation mutagenesis// focused library for the hot-spots positions. | ||
** 6.b List the best predicted substitutions for the hot-spots. | ** 6.b List the best predicted substitutions for the hot-spots. | ||
** 6.c Sketch an in-silico protocol for building a combinatorial library including simultaneous mutations of the hot-spots. | ** 6.c Sketch an in-silico protocol for building a combinatorial library including simultaneous mutations of the hot-spots. | ||
Optional | Optional | ||
=Updates= | =Updates= | ||
