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Maxiprep of plasmid DNA from E. coli: Difference between revisions
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Maxiprep of plasmid DNA from E. coli (view source)
Revision as of 16:07, 17 March 2007
, 17 March 2007→Ingredients
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* 111μL 20mg/mL lysozyme | * 111μL 20mg/mL lysozyme | ||
* 2mL worth of components for solution 2: 200μL 10% SDS, 100μL 4M NaOH, 1.7 mL autoclaved water | * 2mL worth of components for solution 2: 200μL 10% SDS, 100μL 4M NaOH, 1.7 mL autoclaved water | ||
* 1.5mL Solution 3: 3M K<sup>+</sup>, 5M acetate (3M potassium-acetate, 2M acetic acid | * 1.5mL Solution 3: 3M K<sup>+</sup>, 5M acetate (3M potassium-acetate, 2M acetic acid -- glacial is 17M) | ||
* 3.7mL isopropanol | * 3.7mL isopropanol | ||
* 1mL TE buffer | * 1mL TE buffer | ||
