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BME100 s2018:Group9 W0800 L4: Difference between revisions
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<!-- Record all of the materials you will need for PCR --> | <!-- Record all of the materials you will need for PCR --> | ||
* Lab coat and disposable gloves | * Lab coat and disposable gloves | ||
* PCR reacon | * PCR reacon, mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP’s | ||
mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, | |||
(hp://www.promega.com/resources/protocols/product‐informaon‐sheets/g/gotaq‐colorless | (hp://www.promega.com/resources/protocols/product‐informaon‐sheets/g/gotaq‐colorless | ||
‐master‐mix‐m714‐protocol/) | ‐master‐mix‐m714‐protocol/) | ||
* DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes | * DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer | ||
have the same forward primer and reverse primer | |||
* A strip of empty PCR tubes | * A strip of empty PCR tubes | ||
* Disposable pipee | * Disposable pipee | ||
