Shreffler:Notebook/Staff Meetings/2010/08/17

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*Wayne G. Shreffler 09:55, 17 August 2010 (EDT):

Agenda Items

  • EoE
    • EDN ELISAs
      • Sarita has ordered a kit to try? and will initially evaluate with stds from company?
      • Conference call with Mirna on Friday to discuss possible collaboration and availability of samples for validation
    • Component proposal -- WS still needs to work on
  • Basophil
    • Review data from last week
      • non-responder -- x2
      • will implement a 'pre-screen' protocol to avoid basophil isolation and Derp2 specific sensitization with bad donors
      • will run sups for cytokine (CBA) from first 2 exps presuming responder sample obtained this week
      • Review ALK data
        • -- non-responder -- will not save sups
      • Histamine data
        • Background is ~15% -- why so high? donor is non-responder
      • Next experiments
        • Repeat with buffy's this week
  • Allergen Adjuvant
    • 2D done by Galina
      • confirms pI ~10
      • Bert will arrange to have gel sent to Hazmik
    • Foreskin DC flow data -- looked good
      • Thu cells to characterize, Fri Aldefluor
      • Flow Thu 5-7p
    • mAb -- how will we approach
  • PM Baso
    • no samples last week
  • JAX
    • quote for peptide arrays
      • WS has to do
    • train Stephanie for project role
      • Stephanie almost self-sufficient now
  • Carboplatin
    • no samples
  • Isabelle's project -- multi-lingual
    • Action plan available in Spanish, will get Portuguese version
    • Has 30 pages of recipes compiled
  • U19
    • Set up PBMC this week to milk allergen
    • Bert will try to identify allergen source and obtain endotoxin kit

Other Discussion

  • Hood certification/ CO2 tanks
    • will take over cost of that room

Things To Do

  1. Write up component proposal (Marcella and Wayne)
  2. obtain quote for peptide arrays
  3. send Bert U19 slides