Agenda Items
- EoE
- EDN ELISAs
- Sarita has list from Marcella and will order a kit or two to evaluate
- Component proposal -- WS still needs to work on
- Basophil
- Review data from last week (Sarita, Alex sens #7,8)
- Sarita's experiment low baso counts, but good Ag response
- evidence of pre-existing Derp2 sensitization; however, clear evidence of stronger degran with 50:50 sens than 99:1 or 1:99
- Next experiments
- Repeat with buffy's this week, consider lengthening stripping time?
- Caitlin paper? -- Bert has looked?
- Allergen Adjuvant
- Review outcome of meeting w/ S. Carr
- Willing to perform digest/ MS and see
- Review most recent PCR data on dose response to fraction 3
- strong evidence of correlation with prominent band on dilution experiment -- very nice response, much stronger in Fr3 than whole CPE as well
- F/U with Carr
- Bert will take gels over THIS week
- Forski's?
- decent number of CD45+DR+CD1a+, but no evidence of Aledflour enhancement; staining/ flow not optimal
- PM Baso
- JAX
- quote for peptide arrays
- train Stephanie for project role
- U19
- Discuss involvement with Bert
Other Discussion
- Isabelle will see bakery owner on Thu; working on dietary guideline doc, will meet w/ her tomorrow morning
Things To Do
- Investigate EDN assay options/ request samples w/ standards (Sarita)
- Write up component proposal (Marcella and Wayne)
- obtain quote for peptide arrays
- send Bert U19 slides
- send Isabelle Dowling the dining services director name/ contact
|