Shreffler:Notebook/Staff Meetings/2010/05/04

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png ShreffLab Staff Meetings Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Replace with user/time stamp

Agenda Items

  • PM project
    • accumulating data, will analyze after Alex's MCAT
    • MJ will send PM -- Alex will store, stim basos in vitro after MCAT
  • EMAC project
    • samples starting ?this week
  • Cisplatin
    • sample may come tomorrow, but we do not have CD123 in house
      will stain with cocktail -CD123, stain CD123 later if nec
      reviewed first sample together
  • R03
    • trial of CRTH2 staining
      BD Ab at 1 uL inadequate (fresh cells)
    • DHR/ Ca free
      weak response, but supportive of oxidative burst as Ca independent
    • CD63 response almost completely suppressed
    • NEXT:
      DHR titration experiment (draw blood from Alex)
      combined 63/DHR analysis of Ca free (Friday)
  • JAX
    • hold up due to running out of Ab
    • wish to switch to CRTH2 based cocktail
    • T cells
      acquire as is


Previous Action Items


Other Discussion



Things To Do