Shreffler:Notebook/Alex Daily/2011/06/01

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Buhlmann Assay pilot 4

Ran Buhlmann assay using Kadlik buffy, drawn 6/1/11. Used five dilutions each of Milk and Peanut allergen (1.00, 0.20, 0.040, 0.0080, 0.0016 µg/mL)

Followed Buhlmann protocol with following differences:

  • Incubation step in incubator rather than water bath
  • Included anti-IgE Ab positive control


Procedure went great. Especially robust basophil gate and degranulation to positive controls. No response to allergens.