Schmidt Lab:Notebook/Transcriptome Analysis
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Analysis of transcriptome data from Heat Stress Project
Protein Kinase:Substrate List
Used iGEP to identify kinases in complete gene list from LMH cells -included genes not differentially regulated.
Identified a total of 245 kinases in the LMH expression data. Expression of these 217 did not respond to heat stress, 19 were down-regulated and 9 were up-regulated.
Identified all kinases that were either up or down regulated.
PRKCB and PRK2B were the two kinases differentially regulated in both CELC and LMH.
PRKCB Involved in modulation of glucose response.For example: Inhibition of PKCbeta improves glucocorticoid-induced insulin resistance in rat adipocytes . PMID 12665248
PTK2B This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.
PLK1 was down-regulated and had the greatest number of substrates. Used WebGIVI to determine iTerms for PLK1 and its substrates.
From Uniprot: "Polo-like kinase Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. "
MKNK2 Map kinase interacting serine/threonine kinase 2"Eukaryotic initiation factor 4E (eIF4E) controls a crucial step of translation initiation and is critical for cell growth . Biochemical studies have shown that it undergoes a regulated phosphorylation by the MAP-kinase signal-integrating kinases Mnk1 and Mnk2 . Although the role of eIF4E phosphorylation in mammalian cells has remained elusive , recent work in Drosophila has established that it is required for growth and development . Here, we demonstrate that a previously identified Drosophila kinase called Lk6 is the functional homolog of mammalian Mnk kinases. We generated lk6 loss-of-function alleles and found that eIF4E phosphorylation is dramatically reduced in lk6 mutants. Importantly, lk6 mutants exhibit reduced viability, slower development, and reduced adult size, demonstrating that Lk6 function is required for organismal growth."
SIK1 Salt Inducible Kinase"SIK1 represses cAMP-response element (CRE)-mediated transcription of steroidogenic genes,"
Green are down regulated genes while orange are expressed but unchanged by heat stress.