Rosenzweig:Th1 Cell Culture
This is a protocol for the culture of Th1 cells isolated from mouse spleens and lymph nodes.
- 6.5 Thy1.1+/+ mice
- RPMI + 10% FBS
- 10 ng/ml murine IFNgamma
- 25 units/ml murine IL-2
- 5 ng/ml murine IL-12
- 10 μg/ml HA MHC class II peptide
- Scissors and forceps
Isolation of CD4+ T cells
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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Relevant papers and books