Rosenzweig:Th1 Cell Culture

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This is a protocol for the culture of Th1 cells isolated from mouse spleens and lymph nodes.


  • 6.5 Thy1.1+/+ mice
  • CTLM
    • RPMI + 10% FBS
    • 10 ng/ml murine IFNgamma
    • 25 units/ml murine IL-2
    • 5 ng/ml murine IL-12
    • 10 μg/ml HA MHC class II peptide
  • Scissors and forceps


Isolation of CD4+ T cells


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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