Rosenzweig:Th1 Cell Culture

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Overview

This is a protocol for the culture of Th1 cells isolated from mouse spleens and lymph nodes.

Materials

  • 6.5 Thy1.1+/+ mice
  • CTLM
    • RPMI + 10% FBS
    • 10 ng/ml murine IFNgamma
    • 25 units/ml murine IL-2
    • 5 ng/ml murine IL-12
    • 10 μg/ml HA MHC class II peptide
  • Scissors and forceps

Procedure

Isolation of CD4+ T cells

Notes

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References

Relevant papers and books

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