IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/03

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Retrieval of biobricks

  • BBa_K381001 : Plate 4 Well 1A - Nitrate reporter: PyeaR - GFP composite
  • BBa_K325909 : Plate 4 Well 17M - Lux Operon under pBAD
  • 10uL distilled water mixed and incubated 5 mins
  • DNA stored in -20C Parts Registry box

Heat shock transformation of AgrE.Coli and Project Vibrio

Using BBa_K381001 and BBa_K325909 - 0just retrieved. NEB Turbo chemically competent cells prepared 28.6.12 by iGEM team