IGEM:Peking University/2008/Experiment/PKU 080514 G1 1

PKU_080514_G1_1

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PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul

	Volume (μL)
pGREG505/(TEF-lacI-ADHlacS-GFP)	1
easytaq mixture	25	*3
F-Primer	0.5
R-Primer	0.5
ddH2O	23

conditions

Temperature(°C)	Time Length(0:00:00)
94	05:00
94	00:30	30 cycles
50.1,52.2,55.1,58.3,62.2	01:00	30 cycles
72	01:30	30 cycles
72	07:00
Alarm	5 times
Hold	4°C

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