IGEM:Peking University/2008/Experiment/PKU 0701 G2 1
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| Volume (μL) | |
| Template | 1 |
| dNTP | 5 |
| Enzyme | 0.5 |
| Buffer | 5 |
| F-Primer | 0.5 |
| R-Primer | 0.5 |
| ddH2O | 37.5 |
- conditions
| Temperature | Time Length(0:00:00) | |
| 94°C | 02:00 | |
| 94°C | 01:00 | 4 cycles |
| 50°C | 02:00 | 4 cycles |
| 72°C | 05:00 | 4 cycles |
| 94°C | 01:00 | 30 cycles |
| 55.2,55.6,57.6,59.5,61.3,63.9°C | 01:00 | 30 cycles |
| 72°C | 03:00 | 30 cycles |
| 72°C | 10:00 | |
| 4°C | Hold |
- Enzyme cutting-T-Gal4-EcoRI-HindIII-20*4 μL
| Volumn(μL) | |
| DNA | 5 |
| EcoRI | 0.2 |
| HindIII | 0.2 |
| M Buffer | 2 |
| ddH2O | 12.6 |
- Ligation-pUC18/Gal4
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