IGEM:Peking University/2008/Experiment/PKU 0607 G2 1

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TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*6
Volume (μL)
Template (Budding Yeast Genome) 1
dNTP 5
Enzyme 1
Buffer 5
F-Gal4-HindIII-ApaI 1
R-Gal4-EcoRI-Xhol 1
ddH2O 36
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 00:30 4 cycles
94°C 01:00 30 cycles
49.2,52.6,54.6,56.5,58.2,59.8°C 02:00 30 cycles
72°C 04:00 30 cycles
72°C 05:00
4°C Hold

Enzyme cutting-pUC18&Gal4-HindIII-EcoRI-20 μL
Volumn(μL)
pUC18 5
HindIII 0.2
EcoRI 0.2
M Buffer 2
ddH2O 12.6
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