IGEM:Peking University/2008/Experiment/PKU 0607 G2 1

TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*6

	Volume (μL)
Template （Budding Yeast Genome）	1
dNTP	5
Enzyme	1
Buffer	5
F-Gal4-HindIII-ApaI	1
R-Gal4-EcoRI-Xhol	1
ddH2O	36

conditions

Temperature	Time Length(0:00:00)
94°C	02:00
94°C	01:00	4 cycles
50°C	02:00	4 cycles
72°C	00:30	4 cycles
94°C	01:00	30 cycles
49.2,52.6,54.6,56.5,58.2,59.8°C	02:00	30 cycles
72°C	04:00	30 cycles
72°C	05:00
4°C	Hold

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Enzyme cutting-pUC18&Gal4-HindIII-EcoRI-20 μL

	Volumn(μL)
pUC18	5
HindIII	0.2
EcoRI	0.2
M Buffer	2
ddH2O	12.6

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