Matt Gethers/CRI, Thailand/Specifications/pKn001/42.SEQ

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42.SEQ (pKn001.P1, pUC18_rev)

42.SEQ (pKn001.P1, pUC18_rev)

PKn001both.cep

Base Number (Sequence File Annotation) = Contig Sequence File Annotation Number (No trimmed 5' bases)

Base Number (Vector NTI File Annotation) = 434 + Contig Sequence File Annotation Number

Base Number (Sequence File Annotation) Base Number (Vector NTI File Annotation) Theoretical Base Read Base Notes
I'm ignoring through base 34: noisy reading.
240 674 C Space Should be another C - good
386 820 G Space Should be a G - good
388 822 G Space Should be a G - good
400 834 G Space Could be a G - good
413 847 G Space Should be a G - good
441 875 G Space Should be a G - good
456 890 G Space Maybe another G, but not sure
463 897 G Space Should be another G - good
472 906 G Space Should be a G - good
483 917 G Space Doesn't look like there's another G - problem

Notes

Region of Interest: Vector NTI annotation 437 - 1462

Looks good overall - just the last G/Space concerns me.