Matt Gethers/CRI, Thailand/Specifications/pIs001/37.SEQ

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37.SEQ (pIs001.P2, T7_rev repeat)

37.SEQ (pIs001.P2, T7_rev repeat)

PIs001P2contig.cep

Base Number (Sequence File Annotation) = Contig Sequence File Annotation Number + 65 where 64 is the number of trimmed 5' bases (not sure why I have to add 1).

Base Number (Vector NTI File Annotation) = 6108 - Contig Sequence File Annotation Number

Base Number (Sequence File Annotation) Base Number (Vector NTI File Annotation) Theoretical Base Read Base Notes
77 6096 G A Could be a G - good
83 6090 G N Could be an A or G - Not sure
86 6087 G N Could be an A or G - Not sure
130 6043 Space T Definitely an A there - problem
136 6037 C N Looks like a C - good
139 6034 G N Looks like a G - good
140 6033 G T Probably a G - good
141 6032 A N Looks like a A - good
164 6009 C N Looks like a C - good
176 5997 C N C or A - Not sure
177 5996 T N C or T - Not sure
179 5994 Space T Definitely signal for a T there - problem
185 5988 G T Could be a G or a T - Not Sure
187 5986 C N Could be C or G - Not sure
188 5985 G A Could be a G, but strong signal for A - Not Sure
189 5984 G T Could be a G, but could be a T - Not Sure
201 5972 C N Probably a C - good
222 5951 C T Likely a C - good
308 5865 G N Likely a G - good
339 5834 C T Definitely A, no sign of C - problem
379 5794 C N Likely a C - good
393 5780 C N C or A - Not Sure
398 5775 G Space Looks like a G was missed, G also confirmed by other read - good
From 414 (5759) on, there are 5 mismatched spaces, but the base is confirmed by the forward primer - good.

Notes

This read isn't as clean as the forward primer: some points of concern.