Matt Gethers/CRI, Thailand/Specifications/pIs001/36.SEQ

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36.SEQ (pIs001.P1, T7_rev repeat)

36.SEQ (pIs001.P1, T7_rev repeat)

PIs001P1full.cep

Redundant Sequencing: 33.SEQ

Base Number (Sequence File Annotation) = Contig Sequence File Annotation Number + 189 where 189 is the number of trimmed 5' bases.

Base Number (Vector NTI File Annotation) = 5986 - Contig Sequence File Annotation Number

Base Number (Sequence File Annotation) Base Number (Vector NTI File Annotation) Theoretical Base Read Base Notes
202 5974 C N Could be A or C - Not sure
204 5972 G N Could be G or A - Not Sure
223 5953 C T Looks like a C - good
304 5872 C N Maybe a C - Not Sure
309 5867 G N Could be G or T - Not Sure
321 5855 C N Maybe an A or C - Not Sure
333 5843 G N Maybe a G or an A - Not Sure, but G confirmed by other read
342 5834 G A Looks like an A, but could be a G, G confirmed by other read other read
346 5830 G Space Looks like there could be a G there, G confirmed by other read other read

Notes

Region of Interest (Vector NTI Annotation): 5318 - 6121 (ATG to TGA)

Lots of truncated 5' and 3' bases - may want to redo alignment with less trimming/manual comparison. Considering the 189 trimmed 3' bases would allow me to complete the region between the forward and reverse primers (~50 bases between the two) while considering the 188 trimmed 5' bases would allow me to see the end of the ORF (~140 bases to end of ORF).