Matt Gethers/CRI, Thailand/Specifications/pIs001/31.SEQ

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31.SEQ (pIs001.P1, T7_for)

31.SEQ (pIs001.P1, T7_for)

PIs001P1full.cep

PIs001contig.cep - Old alignment; annotations do not refer to this file.

Base Number (Sequence File Annotation) = Contig Sequence File Annotation Number + 29 where 29 is the number of trimmed 5' bases.

Base Number (Vector NTI File Annotation) = 5310 + Contig Sequence File Annotation Number

Base Number (Sequence File Annotation) Base Number (Vector NTI File Annotation) Theoretical Base Read Base Notes
39 5320 G N Looks like a G - good
40 5321 G N Looks like a G - good
78 5359 G T Looks like a T - problem!
81 5361 A C Could be another A - good
82 5262 G C Could be a G - good
83 5263 G N Looks like a G - good
369 5650 C Space Could be a second C - good
391 5672 Space G Insertion of G - problem! Absent the G, sequence from 390 should be CAACCGTGG
394 5675 Space C Could be another C - good
414 5695 G N Could be a G - good
424 5705 G Space Could be a G - good
448 5729 C A Could be a C - good
452 5733 G A Could be a G - good
464 5745 T N Looks like a T - good


Notes

Region of Interest (Vector NTI Annotation): 5318 - 6121 (ATG to TGA)