Matt Gethers/CRI, Thailand/Labwork/Polyacrylamide Gels/Week of 7.7.08
Week of 7.7.08
Poured gel and prepared samples according to protocol. My samples were 1 ml of the 200 ml culture used for isolation of HmgR before induction and 1 ml of the same culture after induction. Because I had only two samples, I loaded, 5, 10, and 20 μl of each.
Gel looks good - protein appears in induced lanes at the correct location (~28 kDa) and not in uninduced lanes. Presence also increases with concentration.
|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5||Lane 6||Lane 7||Lane 8||Lane 9|
|5 μl Unstained protein marker||5 μl uninduced cell sample||5 μl induced cell sample (didn't get into well)||5 μl induced cell sample (did get into well)||10 μl uninduced cell sample||10 μl induced cell sample||20 μl uninduced cell sample||20 μl uninduced cell sample (a little from adjacent lane leaked into well)||20 μl induced cell sample (did get into well)|