Matt Gethers/CRI, Thailand/Labwork/Polyacrylamide Gels/Week of 6.30.08

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Week of 6.30.08

7.3.08

First gel - ran with Mayuree. Made up mix of regents according to her protocol (about 2x). Set the gel 2 days before running it - kept in -4 until used on Thursday. Used Mini Isolation protocol to produce lysate and ran 5 μl in each lane.

Induction looks good. Our band (~28 kDa) is where it should be and only appears significantly in the induced lanes. Presence seems to be balanced between pellet and cytosolic fraction.

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9
5 μl Unstained protein marker 5 μl uninduced cell sample 1, pellet fraction 5 μl induced cell sample 1, pellet fraction 5 μl uninduced cell sample 2, pellet fraction 5 μl induced cell sample 2, pellet fraction 5 μl uninduced cell sample 1, supernatant 5 μl induced cell sample 1, supernatant 5 μl uninduced cell sample 2, supernatant 5 μl induced cell sample 2, supernatant