Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence of Lox/Gen Cassette in pKn004/8.5.08 Gel Results
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8.5.08 Colony PCR Results
| Lane 1 | Lanes 2-6 | Lanes 7 |
| 5 μl λ ladder | 5 μl pKn006.L2 transformant | 5 μl negative control pKn004. |
100 volts, 30 minutes, 1% Agarose gel. 5 μl of product. Stained for 15 minutes. Lanes 2-6 all had bands at ~2.5 kb, but some were slightly off from one another. The negative control worked as expected giving a product at ~1.5 kb.
