Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence of Lox/Gen Cassette in pKn004/8.5.08 Gel Results
8.5.08 Colony PCR Results
|Lane 1||Lanes 2-6||Lanes 7|
|5 μl λ ladder||5 μl pKn006.L2 transformant||5 μl negative control pKn004.|
100 volts, 30 minutes, 1% Agarose gel. 5 μl of product. Stained for 15 minutes. Lanes 2-6 all had bands at ~2.5 kb, but some were slightly off from one another. The negative control worked as expected giving a product at ~1.5 kb.