Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence of Lox/Gen Cassette in pKn004/8.5.08 Gel Results

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8.5.08 Colony PCR Results

Lane 1 Lanes 2-6 Lanes 7
5 μl λ ladder 5 μl pKn006.L2 transformant 5 μl negative control pKn004.

100 volts, 30 minutes, 1% Agarose gel. 5 μl of product. Stained for 15 minutes. Lanes 2-6 all had bands at ~2.5 kb, but some were slightly off from one another. The negative control worked as expected giving a product at ~1.5 kb.