Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18/7.24.08 Master Mix Second Round

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7.24.08 PCR Master Mix For Second Round of Screening (pKn004.L3 Only)

Reagent Volume/Amount
Colony Suspension 0 μl
Taq 1.23 μl
M13 for 9.84 μl 1 μM Stock
BT1188 9.84 μl 1 μM Stock
dNTPs 2.43 (oops, should have been 2.46) μl 10 mM Stock
DMSO 6.15 μl
Buffer 12.3 μl
Mg2+ 6.15 μl
dH20 62.73 μl
Total 122.97 μl (missed 0.03 in dNTPs)

Aliquot 9 μl to each of 12 tubes, then add 1 μl colony suspension to each.