Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18/7.24.08 Master Mix
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7.24.08 PCR Master Mix
| Reagent | Volume/Amount |
| Colony Suspension | 0 μl |
| Taq | 1.63 μl |
| M13 for | 13.04 μl 1 μM Stock |
| BT1188 | 13.04 μl 1 μM Stock |
| dNTPs | 3.26 μl 10 mM Stock |
| DMSO | 8.15 μl |
| Buffer | 16.3 μl |
| Mg2+ | 8.15 μl |
| dH20 | 83.13 μl |
| Total | 146.7 μl |
Aliquot 9 μl to each of 16 tubes, then add 1 μl colony suspension to each.
