Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18/7.24.08 Master Mix

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7.24.08 PCR Master Mix

Reagent Volume/Amount
Colony Suspension 0 μl
Taq 1.63 μl
M13 for 13.04 μl 1 μM Stock
BT1188 13.04 μl 1 μM Stock
dNTPs 3.26 μl 10 mM Stock
DMSO 8.15 μl
Buffer 16.3 μl
Mg2+ 8.15 μl
dH20 83.13 μl
Total 146.7 μl

Aliquot 9 μl to each of 16 tubes, then add 1 μl colony suspension to each.