Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18/7.24.08 Gel Results Second Round
7.24.08 Part II Colony PCR Results
|Lane 1||Lanes 2-6||Lane 7||Lane 8-13|
|5 μl λ ladder||5 μl pKn004.L3 transformant colony PCRs 1-5||5 μl pKn004.L3 transformant colony PCR 6||5 μl pKn004.L3 transformant colony PCRs 7-12|
100 volts, 30 minutes, 1% Agarose gel. 5 μl of product in each lane. Stained for 30 minutes. Everything except lane 7 (again corresponding to colony 6 of the pKn004.L3 suspensions - interesting coincidence) yielded a product at what I think is ~500 bp. Colony 6 is at what I think to be slightly above the ~1500 band, where I expect it assuming a proper integration.