Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18/7.24.08 Gel Results
7.24.08 Colony PCR Results
|Lane 1||Lanes 2-6||Lane 7||Lane 8-9||Lane 10||Lane 11-18|
|5 μl λ ladder||5 μl pKn004.L3 transformant colony PCRs 1-5||5 μl pKn004.L3 transformant colony PCR 6||5 μl pKn004.L3 transformant colony PCRs 7-8||Space||5 μl pKn004.L4 transformant colony PCRs 1-8|
100 volts, 30 minutes, 1% Agarose gel. 5 μl of product in each lane. Stained for 15 minutes. Everything except lane 7 (corresponding to colony 6 of the pKn004.L3 transformation) yielded a product at what I think is ~500 bp. Colony 6 is at what I think to be slightly above the ~1500 band, where I expect it assuming a proper integration.