Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.6.08 Master Mix

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8.3.08 Master Mix

Reagent Volume/Amount
Colony Suspension 16.5 μl (not added to master, though)
Taq 1.65 μl
Primer BT2736 13.2 μl 1 μM Stock
Primer BT2737 13.2 μl 1 μM Stock
dNTPs 3.3 μl 10 mM Stock
DMSO 8.25 μl
Buffer 16.5 μl
Mg2+ 8.25 μl
dH20 84.15 μl
Total 165 μl

Aliquotted 9 μl of to each of 8 tubes, then added 1 μl appropriate colony suspension.