Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.4.08 Master Mix
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8.3.08 Master Mix
| Reagent | Volume/Amount |
| Colony Suspension | 16.5 μl (not added to master, though) |
| Taq | 1.65 μl |
| Primer BT2736 | 13.2 μl 1 μM Stock |
| Primer BT2737 | 13.2 μl 1 μM Stock |
| dNTPs | 3.3 μl 10 mM Stock |
| DMSO | 8.25 μl |
| Buffer | 16.5 μl |
| Mg2+ | 8.25 μl |
| dH20 | 84.15 μl |
| Total | 165 μl |
Aliquotted 9 μl of to each of 8 tubes, then added 1 μl appropriate colony suspension.
