Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.4.08 Master Mix
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8.3.08 Master Mix
Reagent | Volume/Amount |
Colony Suspension | 16.5 μl (not added to master, though) |
Taq | 1.65 μl |
Primer BT2736 | 13.2 μl 1 μM Stock |
Primer BT2737 | 13.2 μl 1 μM Stock |
dNTPs | 3.3 μl 10 mM Stock |
DMSO | 8.25 μl |
Buffer | 16.5 μl |
Mg2+ | 8.25 μl |
dH20 | 84.15 μl |
Total | 165 μl |
Aliquotted 9 μl of to each of 8 tubes, then added 1 μl appropriate colony suspension.